Human TNF-alpha ELISA Kit Vertical Flow

Human TNF-alpha ELISA Kit Vertical FlowProduct overview: Tumor Necrosis Factor alpha (TNF alpha) is a pleiotropic proinflammatory cytokine within the TNF superfamily. The biologically active form of TNF alpha exists as a homotrimer with a molecular mass of 52 kDa, whereas the monomeric form is not biologically active. In humans, TNF alpha is synthesized as a transmembrane protein and assembled intracellularly to form the noncovalently linked homotrimer, which is then expressed on the cell

Cytodiagnostics 100nm NHS-activated Silver Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles

CRP concentrations in plasma change by at least 25% during inflammatory disorders

depending on the biological context

Advantages of Silver Conjugates

Pre-Adsorbed: Yes - Mouse serum adsorbed

Silver nanoparticle core size: 80nm (+/- 3nm)

the spin device is able to retain macromolecules having a greater MW

Absorption max: 410-425nm

assay optimization

Gold nanoparticle core size: 30nm

These features results in reliable and reproducible conjugates

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)